Bio Active Substrate

[gator_frog]

I am building my first tropical arboreal vivarium, and wanted to create a bio active substrate. What's the best way to introduce the right organisms? I have read that adding leaf litter from a clean outdoor site that doesn't include any toxic specimens can do the trick, but I live in New England - will the same organism profile found in the soil and litter content here be useful/safe in a tropical setting? Are there other ways to introduce the right mix into the substrate?

I plan on adding the bio active contents to the following mix:
Orchid bark
Coconut husk chips
Ground coconut husk fiber (expanded)
Sterile vivarium mixture